Detecting m6A methylation regions from Methylated RNA Immunoprecipitation Sequencing.

ABSTRACT:

Motivation

The post-transcriptional epigenetic modification on mRNA is an emerging field to study the gene regulatory mechanism and their association with diseases. Recently developed high-throughput sequencing technology named Methylated RNA Immunoprecipitation Sequencing (MeRIP-seq) enables one to profile mRNA epigenetic modification transcriptome wide. A few computational methods are available to identify transcriptome-wide mRNA modification, but they are either limited by over-simplified model ignoring the biological variance across replicates or suffer from low accuracy and efficiency.

Results

In this work, we develop a novel statistical method, based on an empirical Bayesian hierarchical model, to identify mRNA epigenetic modification regions from MeRIP-seq data. Our m

SUBMITTER: Guo Z 

PROVIDER: S-EPMC9991887 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature