IgG memory B cells expressing IL4R and FCER2 are associated with atopic diseases.

ABSTRACT:

Background

Atopic diseases are characterized by IgE antibody responses that are dependent on cognate CD4 T cell help and T cell-produced IL-4 and IL-13. Current models of IgE cell differentiation point to the role of IgG memory B cells as precursors of pathogenic IgE plasma cells. The goal of this work was to identify intrinsic features of memory B cells that are associated with IgE production in atopic diseases.

Methods

Peripheral blood B lymphocytes were collected from individuals with physician diagnosed asthma or atopic dermatitis (AD) and from non-atopic individuals. These samples were analyzed by spectral flow cytometry, single cell RNA sequencing (scRNAseq), and in vitro activation assays.

Results

We identified a novel population of IgG memory B cells character

SUBMITTER: Aranda CJ 

PROVIDER: S-EPMC9991991 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature