Biotin-dependent cell envelope remodelling is required for Mycobacterium abscessus survival in lung infection.

ABSTRACT: Mycobacterium abscessus is an emerging pathogen causing lung infection predominantly in patients with underlying structural abnormalities or lung disease and is resistant to most frontline antibiotics. As the pathogenic mechanisms of M. abscessus in the context of the lung are not well-understood, we developed an infection model using air-liquid interface culture and performed a transposon mutagenesis and sequencing screen to identify genes differentially required for bacterial survival in the lung. Biotin cofactor synthesis was required for M. abscessus growth due to increased intracellular biotin demand, while pharmacological inhibition of biotin synthesis prevented bacterial proliferation. Biotin was required for fatty acid remodelling, which increased cell envelope fluidity and promote

SUBMITTER: Sullivan MR 

PROVIDER: S-EPMC9992005 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature