Mammalian telomeric RNA (TERRA) can be translated to produce valine-arginine and glycine-leucine dipeptide repeat proteins.

ABSTRACT: Mammalian telomeres consist of (TTAGGG)n repeats. Transcription of the C-rich strand generates a G-rich RNA, termed TERRA, containing G-quadruplex structures. Recent discoveries in several human nucleotide expansion diseases revealed that RNA transcripts containing long runs of 3 or 6 nt repeats which can form strong secondary structures can be translated in multiple frames to generate homopeptide or dipeptide repeat proteins, and multiple studies have shown them to be toxic in cells. We noted that the translation of TERRA would generate two dipeptide repeat proteins: highly charged repeating valine-arginine (VR)_n and hydrophobic repeating glycine-leucine (GL)_n. Here, we synthesized these two dipeptide proteins and raised polyclonal antibodies to VR. The VR dipeptide

SUBMITTER: Al-Turki TM 

PROVIDER: S-EPMC9992779 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature