Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection.

ABSTRACT: Despite its massive potential, Raman imaging represents just a modest fraction of all research and clinical microscopy to date. This is due to the ultralow Raman scattering cross-sections of most biomolecules that impose low-light or photon-sparse conditions. Bioimaging under such conditions is suboptimal, as it either results in ultralow frame rates or requires increased levels of irradiance. Here, we overcome this tradeoff by introducing Raman imaging that operates at both video rates and 1,000-fold lower irradiance than state-of-the-art methods. To accomplish this, we deployed a judicially designed Airy light-sheet microscope to efficiently image large specimen regions. Further, we implemented subphoton per pixel image acquisition and reconstruction to confront issues arising from photo

SUBMITTER: Dunn L 

PROVIDER: S-EPMC9992822 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature