Titin activates myosin filaments in skeletal muscle by switching from an extensible spring to a mechanical rectifier.

ABSTRACT: Titin is a molecular spring in parallel with myosin motors in each muscle half-sarcomere, responsible for passive force development at sarcomere length (SL) above the physiological range (>2.7 μm). The role of titin at physiological SL is unclear and is investigated here in single intact muscle cells of the frog (Rana esculenta), by combining half-sarcomere mechanics and synchrotron X-ray diffraction in the presence of 20 μM para-nitro-blebbistatin, which abolishes the activity of myosin motors and maintains them in the resting state even during activation of the cell by electrical stimulation. We show that, during cell activation at physiological SL, titin in the I-band switches from an SL-dependent extensible spring (OFF-state) to an SL-independent rectifier (ON-state) that allows

SUBMITTER: Squarci C 

PROVIDER: S-EPMC9992839 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature