Conserved reduction of m⁶A RNA modifications during aging and neurodegeneration is linked to changes in synaptic transcripts.

ABSTRACT: N⁶-methyladenosine (m⁶A) regulates mRNA metabolism. While it has been implicated in the development of the mammalian brain and in cognition, the role of m⁶A in synaptic plasticity, especially during cognitive decline, is not fully understood. In this study, we employed methylated RNA immunoprecipitation sequencing to obtain the m⁶A epitranscriptome of the hippocampal subregions CA1, CA3, and the dentate gyrus and the anterior cingulate cortex (ACC) in young and aged mice. We observed a decrease in m⁶A levels in aged animals. Comparative analysis of cingulate cortex (CC) brain tissue from cognitively intact human subjects and Alzheimer's disease (AD) patients showed decreased m⁶A RNA methylation in AD patients. m⁶A

SUBMITTER: Castro-Hernandez R 

PROVIDER: S-EPMC9992849 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature