Expression and function of an S1-type nuclease in the digestive fluid of a sundew, Drosera adelae.

ABSTRACT:

Background and aims

Carnivorous plants trap and digest insects and similar-sized animals. Many studies have examined enzymes in the digestive fluids of these plants and have gradually unveiled the origins and gene expression of these enzymes. However, only a few attempts have been made at characterization of nucleases. This study aimed to reveal gene expression and the structural, functional and evolutionary characteristics of an S1-type nuclease (DAN1) in the digestive fluid of an Australian sundew, Drosera adelae, whose trap organ shows unique gene expression and related epigenetic regulation.

Methods

Organ-specificity in Dan1 expression was examined using glandular tentacles, laminas, roots and inflorescences, and real-time PCR. The methylation status of the Dan1 promoter

SUBMITTER: Yu M 

PROVIDER: S-EPMC9992940 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature