Concurrent Vibrational Circular Dichroism Measurements with Infrared Spectroscopic Imaging.

ABSTRACT: Vibrational circular dichroism (VCD) spectroscopy has emerged as a powerful platform to quantify chirality, a vital biological property that performs a pivotal role in the metabolism of life organisms. With a photoelastic modulator (PEM) integrated into an infrared spectrometer, the differential response of a sample to the direction of circularly polarized light can be used to infer conformation handedness. However, these optical components inherently exhibit chromatic behavior and are typically optimized at discrete spectral frequencies. Advancements of discrete frequency infrared (DFIR) spectroscopic microscopes in spectral image quality and data throughput are promising for use toward analytical VCD measurements. Utilizing the PEM advantages incorporated into a custom-built QCL microsco

SUBMITTER: Phal Y 

PROVIDER: S-EPMC9993326 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature