Mitochondrial metabolism of the facultative parasite Chilodonella uncinata (Alveolata, Ciliophora).

ABSTRACT:

Background

Chilodonella uncinata is an aerobic ciliate capable of switching between being free-living and parasitic on fish fins and gills, causing tissue damage and host mortality. It is widely used as a model organism for genetic studies, but its mitochondrial metabolism has never been studied. Therefore, we aimed to describe the morphological features and metabolic characteristics of its mitochondria.

Methods

Fluorescence staining and transmission electron microscopy (TEM) were used to observe the morphology of mitochondria. Single-cell transcriptome data of C. uncinata were annotated by the Clusters of Orthologous Genes (COG) database. Meanwhile, the metabolic pathways were constructed based on the transcriptomes. The phylogenetic analysis was also made based on the sequ

SUBMITTER: Bu XL 

PROVIDER: S-EPMC9993649 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature