Arabidopsis thaliana NudiXes have RNA-decapping activity.

ABSTRACT: Recent discoveries of various noncanonical RNA caps, such as dinucleoside polyphosphates (Np _n N), coenzyme A (CoA), and nicotinamide adenine dinucleotide (NAD) in all domains of life have led to a revision of views on RNA cap function and metabolism. Enzymes from the NudiX family capable of hydrolyzing a polyphosphate backbone attached to a nucleoside are the strongest candidates for degradation of noncanonically capped RNA. The model plant organism Arabidopsis thaliana encodes as many as 28 NudiX enzymes. For most of them, only in vitro substrates in the form of small molecules are known. In our study, we focused on four A. thaliana NudiX enzymes (AtNUDT6, AtNUDT7, AtNUDT19 and AtNUDT27), and we studied whether these enzymes can cleave RNA capped wi

SUBMITTER: Mititelu MB 

PROVIDER: S-EPMC9994101 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature