Enzyme expression kinetics by Escherichia coli during transition from rich to minimal media depends on proteome reserves.

ABSTRACT: Bacterial fitness depends on adaptability to changing environments. In rich growth medium, which is replete with amino acids, Escherichia coli primarily expresses protein synthesis machineries, which comprise ~40% of cellular proteins and are required for rapid growth. Upon transition to minimal medium, which lacks amino acids, biosynthetic enzymes are synthesized, eventually reaching ~15% of cellular proteins when growth fully resumes. We applied quantitative proteomics to analyse the timing of enzyme expression during such transitions, and established a simple positive relation between the onset time of enzyme synthesis and the fractional enzyme 'reserve' maintained by E. coli while growing in rich media. We devised and validated a coarse-grained kinetic model that quantitatively capture

SUBMITTER: Wu C 

PROVIDER: S-EPMC9994330 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature