Molecular characterization of Helicobacter pylori clinical isolates from Eastern Saudi Arabia.

ABSTRACT:

Objectives

To describe the frequency of cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA) virulence genes and clarithromycin resistance-associated mutations among Helicobacter pylori (H. pylori) clinical isolates from Eastern Saudi Arabia.

Methods

A cross-sectional study was carried out between July 2020 and June 2021 in a tertiary hospital in AL-Khobar, Saudi Arabia. A total of 34 H. pylori isolates were obtained from gastric biopsies of patients with dyspepsia. The existence of the virulence genes was studied by polymerase chain reaction and the gene fragment of the 23s ribosomal subunit (23s rRNA) gene was sequenced.

Results

All isolates harbored the CagA gene. Approximately 9

SUBMITTER: Alkharsah KR 

PROVIDER: S-EPMC9994491 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature