HPLC-Parallel accelerator and molecular mass spectrometry analysis of ¹⁴C-labeled amino acids.

ABSTRACT: Accelerator mass spectrometry (AMS) is the method of choice for quantitation of low amounts of ¹⁴C-labeled biomolecules. Despite exquisite sensitivity, an important limitation of AMS is its inability to provide structural information about the analyte. This limitation is not critical when the labeled compounds are well-characterized prior to AMS analysis. However, analyte identity is important in other experiments where, for example, a compound is metabolized and the structures of its metabolites are not known. We previously described a moving wire interface that enables direct AMS measurement of liquid sample in the form of discrete drops or HPLC eluent without the need for individual fraction collection, termed liquid sample-AMS (LS-AMS). We now report the coupling of LS-AMS w

SUBMITTER: Baliu-Rodriguez D 

PROVIDER: S-EPMC9994536 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature