Longitudinal characterization of circulating extracellular vesicles and small RNA during simian immunodeficiency virus infection and antiretroviral therapy.

ABSTRACT:

Objectives

Latent infection by human immunodeficiency virus (HIV) hinders viral eradication despite effective antiretroviral treatment (ART). Amongst proposed contributors to viral latency are cellular small RNAs that have also been proposed to shuttle between cells in extracellular vesicles (EVs). Thus, we profiled EV small RNAs during different infection phases to understand the potential relationship between these EV-associated small RNAs and viral infection.

Design

A well characterized simian immunodeficiency virus (SIV)/macaque model of HIV was used to profile EV-enriched blood plasma fractions harvested during pre-infection, acute infection, latent infection/ART treatment, and rebound after ART interruption.

Methods

Measurement of EV concentration, size distribu

SUBMITTER: Huang Y 

PROVIDER: S-EPMC9994802 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature