Dynamic bioinspired coculture model for probing ER⁺ breast cancer dormancy in the bone marrow niche.

ABSTRACT: Late recurrences of breast cancer are hypothesized to arise from disseminated tumor cells (DTCs) that reactivate after dormancy and occur most frequently with estrogen receptor-positive (ER⁺) breast cancer cells (BCCs) in bone marrow (BM). Interactions between the BM niche and BCCs are thought to play a pivotal role in recurrence, and relevant model systems are needed for mechanistic insights and improved treatments. We examined dormant DTCs in vivo and observed DTCs near bone lining cells and exhibiting autophagy. To study underlying cell-cell interactions, we established a well-defined, bioinspired dynamic indirect coculture model of ER⁺ BCCs with BM niche cells, human mesenchymal stem cells (hMSCs) and fetal osteoblasts (hFOBs). hMSCs promoted BCC growth, whereas h

SUBMITTER: Pradhan L 

PROVIDER: S-EPMC9995072 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature