Intravital imaging-based genetic screen reveals the transcriptional network governing Candida albicans filamentation during mammalian infection.

ABSTRACT: Candida albicans is one of the most common human fungal pathogens. C. albicans pathogenesis is tightly linked to its ability to under a morphogenetic transition from typically budding yeast to filamentous forms of hyphae and pseudohyphae. Filamentous morphogenesis is the most intensively studied C. albicans virulence traits; however, nearly all of these studies have been based on in vitro induction of filamentation. Using an intravital imaging assay of filamentation during mammalian (mouse) infection, we have screened a library of transcription factor mutants to identify those that modulate both the initiation and maintenance of filamentation in vivo. We coupled this initial screen with genetic interaction analysis and in vivo transcription profiling to characterize th

SUBMITTER: Wakade RS 

PROVIDER: S-EPMC9995110 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature