CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization.

ABSTRACT: Multilocular adipocytes are a hallmark of thermogenic adipose tissue^1,2, but the factors that enforce this cellular phenotype are largely unknown. Here, we show that an adipocyte-selective product of the Clstn3 locus (CLSTN3β) present in only placental mammals facilitates the efficient use of stored triglyceride by limiting lipid droplet (LD) expansion. CLSTN3β is an integral endoplasmic reticulum (ER) membrane protein that localizes to ER-LD contact sites through a conserved hairpin-like domain. Mice lacking CLSTN3β have abnormal LD morphology and altered substrate use in brown adipose tissue, and are more susceptible to cold-induced hypothermia despite having no defect in adrenergic signalling. Conversely, forced expression of CLSTN3β is sufficient to enforce a multilocular LD

SUBMITTER: Qian K 

PROVIDER: S-EPMC9995219 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature