Canine peripheral blood TCRαβ T cell atlas: Identification of diverse subsets including CD8A⁺ MAIT-like cells by combined single-cell transcriptome and V(D)J repertoire analysis.

ABSTRACT: The dog is valued as a companion animal and increasingly recognized as a model for human disorders. Given the importance of T cells in health and disease, comprehensive knowledge of canine T cells can contribute to our understanding of pathogenesis mechanisms and inform the development of new treatment strategies. However, the diversity of canine T cells is still poorly understood mainly due to the lack of species-reactive antibodies for use in flow cytometry. The aim of this study was to generate a detailed atlas of peripheral blood TCRαβ⁺ T cells of healthy dogs using single-cell RNA-sequencing (scRNAseq) combined with immune repertoire sequencing. A total of 22 TCRαβ⁺ T cell clusters were identified, which were classified into three major groups: CD4-dominant (11 c

SUBMITTER: Eschke M 

PROVIDER: S-EPMC9995359 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature