Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers.

ABSTRACT: Microfluidic cultivation, with its high level of environmental control and spatio-temporal resolution of cellular behavior, is a well-established tool in today's microfluidics. Yet, reliable retention of (randomly) motile cells inside designated cultivation compartments still represents a limitation, which prohibits systematic single-cell growth studies. To overcome this obstacle, current approaches rely on complex multilayer chips or on-chip valves, which makes their application for a broad community of users infeasible. Here, we present an easy-to-implement cell retention concept to withhold cells inside microfluidic cultivation chambers. By introducing a blocking structure into a cultivation chamber's entrance and nearly closing it, cells can be manually pushed into the chamber during l

SUBMITTER: Schmitz J 

PROVIDER: S-EPMC9995442 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature