Adherent-invasive E. coli - induced specific IgA limits pathobiont localization to the epithelial niche in the gut.

ABSTRACT:

Background and aim

Adherent-invasive E. coli (AIEC) has been identified as a pathobiont associated with Crohn's disease (CD), that prefers to grow in inflammatory conditions. Although the colonization by AIEC is implicated in the progression of the disease and exacerbates inflammation in murine colitis models, the recognition and response of host immunity to AIEC remains elusive.

Methods

Antibiotic treated female C57BL/6 mice were inoculated by commensal E. coli and LF82 AIEC strains. Luminal-IgA fractions were prepared from feces and their binding to AIEC and other strains was assessed to confirm specificity. IgA binding to isogenic mutant strains was performed to identify the functional molecules that are recognized by AIEC specific IgA. The effect of IgA on

SUBMITTER: Tanaka R 

PROVIDER: S-EPMC9995611 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature