Anti-fibrotic effect of tocotrienols for bladder dysfunction due to partial bladder outlet obstruction.

ABSTRACT:

Purpose

To investigate potential beneficial effects of tocotrienols which have been suggested to inhibit hypoxia-inducible factor (HIF) pathway, on partial bladder outlet obstruction (PBOO)-induced bladder pathology.

Materials and methods

PBOO was surgically created in juvenile male mice. Sham-operated mice were used as controls. Animals received daily oral administration of either tocotrienols (T₃) or soybean oil (SBO, vehicle) from day 0 to 13 post-surgery. Bladder function was examined in vivo by void spot assay. At 2 weeks post-surgery, the bladders were subjected to physiological evaluation of detrusor contractility in vitro using bladder strips, histology by H&E staining and collagen imaging, and gene expression analyses by quantitative PCR

SUBMITTER: Iguchi N 

PROVIDER: S-EPMC9995959 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature