Characterization of hexokinase gene family members in Glycine max and functional analysis of GmHXK2 under salt stress.

ABSTRACT: Hexokinase (HXK) is a bifunctional enzyme involved in carbohydrate metabolism and sugar signal sensing. HXK gene family has been extensively discussed in many species, while the detailed investigations of the family in Glycine max have yet to be reported. In this study, 17 GmHXK genes (GmHXKs) were identified in the G. max genome and the features of their encoded proteins, conserved domains, gene structures, and cis-acting elements were systematically characterized. The GmHXK2 gene isolated from G. max was firstly constructed into plant expression vector pMDC83 and then transformed with Agrobacterium tumefaciens into Arabidopsis thaliana. The expression of integrated protein was analyzed by Western Blotting. Subcellular localization a

SUBMITTER: Chen S 

PROVIDER: S-EPMC9996050 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature