Investigating Klebsiella pneumoniae biofilm preservation for scanning electron microscopy.

ABSTRACT: Klebsiella pneumoniae biofilm formation is associated with chronic and relapsing infections. Scanning electron microscopy (SEM) is a powerful tool for characterizing biofilm structure and studying their formation. Reliable visualization of biofilm structure requires careful sample preservation, otherwise there may be loss of non-covalent interactions that are susceptible to damage during the dehydration and washing preparation steps. However, no standard procedure has been adopted in the literature to fix K. pneumoniae biofilm for scanning electron microscopy studies. This lack of standardization makes it challenging to compare results between studies and determine the degree to which native structures have been preserved. To advance this critical area of study, we investigat

SUBMITTER: Fleeman RM 

PROVIDER: S-EPMC9996182 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature