One-shot ¹³ C¹⁵ N-metabolic flux analysis for simultaneous quantification of carbon and nitrogen flux.

ABSTRACT: Metabolic flux is the final output of cellular regulation and has been extensively studied for carbon but much less is known about nitrogen, which is another important building block for living organisms. For the tuberculosis pathogen, this is particularly important in informing the development of effective drugs targeting the pathogen's metabolism. Here we performed ¹³ C¹⁵ N dual isotopic labeling of Mycobacterium bovis BCG steady state cultures, quantified intracellular carbon and nitrogen fluxes and inferred reaction bidirectionalities. This was achieved by model scope extension and refinement, implemented in a multi-atom transition model, within the statistical framework of Bayesian model averaging (BMA). Using BMA-based ¹³ C¹⁵ N-metabolic fl

SUBMITTER: Borah Slater K 

PROVIDER: S-EPMC9996240 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature