Influence of glycoprotein MUC1 on trafficking of the Ca²⁺-selective ion channels, TRPV5 and TRPV6, and on in vivo calcium homeostasis.

ABSTRACT: Polymorphism of the gene encoding mucin 1 (MUC1) is associated with skeletal and dental phenotypes in human genomic studies. Animals lacking MUC1 exhibit mild reduction in bone density. These phenotypes could be a consequence of modulation of bodily Ca homeostasis by MUC1, as suggested by the previous observation that MUC1 enhances cell surface expression of the Ca²⁺-selective channel, TRPV5, in cultured unpolarized cells. Using biotinylation of cell surface proteins, we asked whether MUC1 influences endocytosis of TRPV5 and another Ca²⁺-selective TRP channel, TRPV6, in cultured polarized epithelial cells. Our results indicate that MUC1 reduces endocytosis of both channels, enhancing cell surface expression. Further, we found that mice lacking MUC1 lose apical localiz

SUBMITTER: Al-Bataineh MM 

PROVIDER: S-EPMC9996365 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature