Tracking the progeny of adoptively transferred virus-specific T cells in patients posttransplant using TCR sequencing.

ABSTRACT: Adoptive cellular therapies with T cells are increasingly used to treat a variety of conditions. For instance, in a recent phase 1/2 trial, we prophylactically administered multivirus-specific T-cell products to protect recipients of T-cell-depleted allogeneic stem cell grafts against viral reactivation. To establish treatment efficacy, it is important to determine the fate of the individual transferred T-cell populations. However, it is difficult to unequivocally distinguish progeny of the transferred T-cell products from recipient- or stem cell graft-derived T cells that survived T-cell depletion during conditioning or stem cell graft manipulation. Using messenger RNA sequencing of the T-cell receptor β-chains of the individual virus-specific T-cell populations within these T-cell produc

SUBMITTER: Huisman W 

PROVIDER: S-EPMC9996373 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature