LC-MS-Based Targeted Metabolomics for FACS-Purified Rare Cells.

ABSTRACT: Metabolism plays a fundamental role in regulating cellular functions and fate decisions. Liquid chromatography-mass spectrometry (LC-MS)-based targeted metabolomic approaches provide high-resolution insights into the metabolic state of a cell. However, the typical sample size is in the order of 10⁵-10⁷ cells and thus not compatible with rare cell populations, especially in the case of a prior flow cytometry-based purification step. Here, we present a comprehensively optimized protocol for targeted metabolomics on rare cell types, such as hematopoietic stem cells and mast cells. Only 5000 cells per sample are required to detect up to 80 metabolites above background. The use of regular-flow liquid chromatography allows for robust data acquisition, and the omission of dr

SUBMITTER: Schonberger K 

PROVIDER: S-EPMC9996616 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature