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A curcumin direct protein biosensor for cell-free prototyping.

ABSTRACT: In synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an Escherichia coli double bond reductase (EcCurA), as a detection unit with its substrate curcumin-we call this a direct protein (DiPro) biosensor. Using a cell-free synthetic biology approach, we use the EcCurA DiPro biosensor to fine tune 10 reaction parameters (cofactor, substrate, and enzyme levels) for cell-free curcumin biosynthesis, assisted through acoustic liquid handling robotics. Overall, we increase EcCurA-curcumin DiPro fluorescence within cell-free reactions by 78-fold. This finding adds to the growing family of protein-ligand complexes that are naturally fluorescent and potentially exploitable for a range of applications, including medical imaging to engineering high-value chemicals.

SUBMITTER: Kennedy A

PROVIDER: S-EPMC9996706 | biostudies-literature | 2022 Jun-Sep

REPOSITORIES: biostudies-literature

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A curcumin direct protein biosensor for cell-free prototyping.

Kennedy Agata A Griffin Guy G Freemont Paul S PS Polizzi Karen M KM Moore Simon J SJ

Engineering biology 20220601 2-3

In synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an Escherichia coli double bond reductase (EcCurA), as a detection unit with its substrate curcumin-we call this a direct protein (DiPro) biosensor. Using a cell-free synthetic biology approach, we use the EcCurA DiPro biosensor to fine tune 10 reaction parameters (cofactor, subst ...[more]

PMID: 36969103

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