Identifying Eukaryotes and Factors Influencing Their Biogeography in Drinking Water Metagenomes.

ABSTRACT: The biogeography of eukaryotes in drinking water systems is poorly understood relative to that of prokaryotes or viruses, limiting the understanding of their role and management. A challenge with studying complex eukaryotic communities is that metagenomic analysis workflows are currently not as mature as those that focus on prokaryotes or viruses. In this study, we benchmarked different strategies to recover eukaryotic sequences and genomes from metagenomic data and applied the best-performing workflow to explore the factors affecting the relative abundance and diversity of eukaryotic communities in drinking water distribution systems (DWDSs). We developed an ensemble approach exploiting k-mer- and reference-based strategies to improve eukaryotic sequence identification and identifi

SUBMITTER: Gabrielli M 

PROVIDER: S-EPMC9996835 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature