DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation.

ABSTRACT:

Background

Knowledge about regulating transcription factors (TFs) for osteoblastogenesis from mesenchymal stem cells (MSCs) is limited. Therefore, we investigated the relationship between genomic regions subject to DNA-methylation changes during osteoblastogenesis and the TFs known to directly interact with these regulatory regions.

Results

The genome-wide DNA-methylation signature of MSCs differentiated to osteoblasts and adipocytes was determined using the Illumina HumanMethylation450 BeadChip array. During adipogenesis no CpGs passed our test for significant methylation changes. Oppositely, during osteoblastogenesis we identified 2462 differently significantly methylated CpGs (adj. p < 0.05). These resided outside of CpGs islands and were significantly enriched in enhance

SUBMITTER: Gomez R 

PROVIDER: S-EPMC9996951 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature