Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3.

ABSTRACT:

Background

Mechanical therapies, such as distraction osteogenesis, are widely used in dental clinics. During this process, the mechanisms by which tensile force triggers bone formation remain of interest. Herein, we investigated the influence of cyclic tensile stress on osteoblasts and identified the involvement of ERK1/2 and STAT3.

Materials and methods

Rat clavarial osteoblasts were subjected to tensile loading (10% elongation, 0.5 Hz) for different time periods. RNA and protein levels of osteogenic markers were determined using qPCR and western blot after inhibition of ERK1/2 and STAT3. ALP activity and ARS staining revealed osteoblast mineralization capacity. The interaction between ERK1/2 and STAT3 was investigated by immunofluorescence, western blot, and Co-IP.

Resu

SUBMITTER: Xiao X 

PROVIDER: S-EPMC9996996 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature