Differentiation of advanced generation mutant wheat lines: Conventional techniques versus Raman spectroscopy.

ABSTRACT: This research aimed to assess the feasibility of utilizing Raman spectroscopy in plant breeding programs. For this purpose, the evaluation of the mutant populations set up the application of 4 mM NaN₃ to the somatic embryos obtained from mature wheat (Triticum aestivum L. Adana-99 cv.) embryos. Advanced wheat mutant lines, which were brought up to the seventh generation with salt stress tolerance by following in vitro and in vivo environments constructed by mutated populations, were evaluated using conventional techniques [measurement of antioxidant enzyme activities (SOD, CAT, and POX), total chlorophyll, TBARS, and proline contents; measurement of the concentration of Na⁺ and K⁺ ions; and evaluation of gene expression by qPCR (Ta</

SUBMITTER: Sen A 

PROVIDER: S-EPMC9997642 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature