The small GTPase ARF3 controls invasion modality and metastasis by regulating N-cadherin levels.

ABSTRACT: ARF GTPases are central regulators of membrane trafficking that control local membrane identity and remodeling facilitating vesicle formation. Unraveling their function is complicated by the overlapping association of ARFs with guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and numerous interactors. Through a functional genomic screen of three-dimensional (3D) prostate cancer cell behavior, we explore the contribution of ARF GTPases, GEFs, GAPs, and interactors to collective invasion. This revealed that ARF3 GTPase regulates the modality of invasion, acting as a switch between leader cell-led chains of invasion or collective sheet movement. Functionally, the ability of ARF3 to control invasion modality is dependent on association and subsequent control of tu

SUBMITTER: Sandilands E 

PROVIDER: S-EPMC9997661 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature