Large surface deformation due to thermo-mechanical effects during cryopreservation by vitrification - mathematical model and experimental validation.

ABSTRACT: This study presents a simplified thermal-fluids (TF) mathematical model to analyze large surface deformations in cryoprotective agents (CPA) during cryopreservation by vitrification. The CPA deforms during vitrification due to material flow caused by the combined effects of thermal gradients within the domain, thermal contraction due to temperature, and exponential increase in the viscosity of the CPA as it is cooled towards glass transition. While it is well understood that vitrification is associated with thermo-mechanical stress, which might lead to structural damage, those large deformations can lead to stress concentration, further intensifying the probability to structural failure. The results of the TF model are experimentally validated by means of cryomacroscopy on a cuvette contai

SUBMITTER: Vispute DM 

PROVIDER: S-EPMC9997942 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature