Targeting CD38 to Suppress Osteoarthritis Development and Associated Pain After Joint Injury in Mice.

ABSTRACT:

Objective

This study was undertaken to determine the role of CD38, which can function as an enzyme to degrade NAD⁺ , in osteoarthritis (OA) development.

Methods

Human knee cartilage from normal donors and OA donors were examined for CD38 expression. "Gain-of-function," through overexpression of CD38 via transient transfection, and "loss-of-function," through pharmacologic inhibition of CD38, approaches were used to assess the effects of CD38 on intracellular NAD⁺ :NADH ratio and catabolic activity in chondrocytes. We also initiated joint injury-induced OA by surgical destabilization of the medial meniscus (DMM) in CD38 knockout mice and wild-type (WT; C57BL/6) mice and in WT male mice in the presence or absence of apigenin treatment. Cartilage degradati

SUBMITTER: Gil Alabarse P 

PROVIDER: S-EPMC9998345 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature