Single-cell RNA sequencing reveals cell landscape following antimony exposure during spermatogenesis in Drosophila testes.

ABSTRACT: Antimony (Sb), is thought to induce testicular toxicity, although this remains controversial. This study investigated the effects of Sb exposure during spermatogenesis in the Drosophila testis and the underlying transcriptional regulatory mechanism at single-cell resolution. Firstly, we found that flies exposed to Sb for 10 days led to dose-dependent reproductive toxicity during spermatogenesis. Protein expression and RNA levels were measured by immunofluorescence and quantitative real-time PCR (qRT-PCR). Single-cell RNA sequencing (scRNA-seq) was performed to characterize testicular cell composition and identify the transcriptional regulatory network after Sb exposure in Drosophila testes. scRNA-seq analysis revealed that Sb exposure influenced various testicular cell populations, especia

SUBMITTER: Yu J 

PROVIDER: S-EPMC9998446 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature