Genes Vary Greatly in Their Propensity for Collateral Fitness Effects of Mutations.

ABSTRACT: Mutations can have deleterious fitness effects when they decrease protein specific activity or decrease active protein abundance. Mutations will also be deleterious when they cause misfolding or misinteractions that are toxic to the cell (i.e., independent of whether the mutations affect specific activity and abundance). The extent to which protein evolution is shaped by these and other collateral fitness effects is unclear in part because little is known of their frequency and magnitude. Using deep mutational scanning (DMS), we previously found at least 42% of missense mutations in the TEM-1 β-lactamase antibiotic resistance gene cause deleterious collateral fitness effects. Here, we used DMS to comprehensively determine the collateral fitness effects of missense mutations in three genes

SUBMITTER: Mehlhoff JD 

PROVIDER: S-EPMC9999109 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature