Selectable encapsulated cell quantity in droplets via label-free electrical screening and impedance-activated sorting.

ABSTRACT: Single-cell encapsulation in droplets has become a powerful tool in immunotherapy, medicine discovery, and single-cell analysis, thanks to its capability for cell confinement in picoliter volumes. However, the purity and throughput of single-cell droplets are limited by random encapsulation process, which resuts in a majority of empty and multi-cells droplets. Herein we introduce the first label-free selectable cell quantity encapsulation in droplets sorting system to overcome this problem. The system utilizes a simple and reliable electrical impedance based screening (98.9% of accuracy) integrated with biocompatible acoustic sorting to select single-cell droplets, achieving 90.3% of efficiency and up to 200 ​Hz of throughput, by removing multi-cells (∼60% of rejection) and empty droplets

SUBMITTER: Zhong J 

PROVIDER: S-EPMC9999206 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature