Multiplex gel-based PCR assay for the simultaneous detection of 5 genotypes of porcine astroviruses.

ABSTRACT: Porcine astrovirus (PAstV) has been associated experimentally with diarrhea in piglets, but much more knowledge is needed about this virus. PAstV has high genetic variability, and 5 genotypes have been identified, namely PAstV1-5. To obtain information on the epidemiology of PAstV, we established a multiplex PAstV PCR assay to detect and differentiate the 5 PAstV genotypes simultaneously. The assay utilized specific primers for each genotype, producing fragments of 307, 353, 205, 253, and 467 bp, representing PAstV1-5, respectively. Our multiplex PCR assay amplified all 5 DNA fragments from single or mixed viral genomes without cross-reactions with other PAstV genotypes or other viruses in pigs. The limit of detection of the multiplex PCR assay was 5 × 10² copies/μL for PAstV1 a

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC9999398 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature