Extracellular vesicle distribution and localization in skeletal muscle at rest and following disuse atrophy.

ABSTRACT:

Background

Skeletal muscle (SkM) is a large, secretory organ that produces and releases myokines that can have autocrine, paracrine, and endocrine effects. Whether extracellular vesicles (EVs) also play a role in the SkM adaptive response and ability to communicate with other tissues is not well understood. The purpose of this study was to investigate EV biogenesis factors, marker expression, and localization across cell types in the skeletal muscle. We also aimed to investigate whether EV concentrations are altered by disuse atrophy.

Methods

To identify the potential markers of SkM-derived EVs, EVs were isolated from rat serum using density gradient ultracentrifugation, followed by fluorescence correlation spectroscopy measurements or qPCR. Single-cell RNA sequencing (scRNA

SUBMITTER: Ismaeel A 

PROVIDER: S-EPMC9999658 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature