Dataset Information

Whole Genome Metabolism - Aster yellows witches'-broom phytoplasma (strain AYWB)

ABSTRACT:

Whole Genome Metabolism of "Aster yellows witches'-broom phytoplasma (strain AYWB)"

This is a whole genome metabolism model of Aster yellows witches'-broom phytoplasma (strain AYWB).

This model has been automatically generated by the SuBliMinaL Toolbox and libAnnotationSBML using information coming from from KEGG (release 66, April 2013, accessed via the resource's web services interface) and, where relevant, augmented with metabolic pathway information extracted from MetaCyc (version 17.0, March 2013).

This model has been produced by the path2models project and is currently hosted on BioModels Database and identified by: BMID000000140978 .

Other models with the same genus include BMID000000018758 BMID000000018759 BMID000000018760 BMID000000018761 BMID000000018762 BMID000000018763 BMID000000018764 BMID000000018765 BMID000000018766 BMID000000018767 BMID000000018768 BMID000000018769 BMID000000018770 BMID000000104373 BMID000000104374 BMID000000104375 BMID000000104376 BMID000000104377 BMID000000104378 BMID000000104379 BMID000000104380 BMID000000104381 BMID000000104382 BMID000000104383 BMID000000104384 BMID000000104385 BMID000000104386 BMID000000104387 BMID000000104388 BMID000000104389 BMID000000104390 BMID000000104391 BMID000000104392 BMID000000104393 BMID000000104394 BMID000000104395 .

To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

SUBMITTER: Path2Models project

PROVIDER: BMID000000140978 | biostudies-other |

SECONDARY ACCESSION(S): 24180668

REPOSITORIES: biostudies-other

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Publications

Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts.

Bai Xiaodong X Zhang Jianhua J Ewing Adam A Miller Sally A SA Jancso Radek Agnes A Shevchenko Dmitriy V DV Tsukerman Kiryl K Walunas Theresa T Lapidus Alla A Campbell John W JW Hogenhout Saskia A SA

Journal of bacteriology 20060501 10

Phytoplasmas ("Candidatus Phytoplasma," class Mollicutes) cause disease in hundreds of economically important plants and are obligately transmitted by sap-feeding insects of the order Hemiptera, mainly leafhoppers and psyllids. The 706,569-bp chromosome and four plasmids of aster yellows phytoplasma strain witches' broom (AY-WB) were sequenced and compared to the onion yellows phytoplasma strain M (OY-M) genome. The phytoplasmas have small repeat-rich genomes. This comparative analysis revealed ...[more]

PMID: 16672622

Publication: 1/2

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Project description:tRNAs are encoded by a large gene family, usually with several isogenic tRNAs interacting with the same codon. Mutations in the anticodon region of other tRNAs can overcome specific tRNA deficiencies. Phylogenetic analysis suggests that such mutations have occurred in evolution, but the driving force is unclear. We show that in yeast suppressor mutations in other tRNAs are able to overcome deficiency of the essential TRT2-encoded tRNAThrCGU at high temperature (40°C). Surprisingly, these tRNA suppressor mutations were obtained after whole-genome transformation with DNA from thermotolerant Kluyveromyces marxianus or Ogataea polymorpha strains, but from which the mutations did apparently not originate. We suggest that transient presence of donor DNA in the host facilitates proliferation at high temperature and thus increases the chances for occurrence of spontaneous mutations suppressing defective growth at high temperature. Whole-genome sequence analysis of three transformants revealed only four to five non-synonymous mutations of which one causing TRT2 anticodon stem stabilization and two anticodon mutations in non-threonyl-tRNAs, tRNALysCUU and tRNAeMetCAU, were causative. Both anticodon mutations suppressed lethality of TRT2 deletion and apparently caused the respective tRNAs to become novel substrates for threonyl-tRNA synthetase. LC-MS/MS data could not detect any significant mistranslation and RT-qPCR results contradicted induction of the unfolded protein response. We suggest that stress conditions have been a driving force in evolution for the selection of anticodon-switching mutations in tRNAs as revealed by phylogenetic analysis. Importance of the work In this work we have identified for the first time the causative elements in a eukaryotic organism introduced by applying whole-genome transformation and responsible for the selectable trait of interest, i.e. high temperature tolerance. Surprisingly, the whole-genome transformants contained just a few SNPs, which were unrelated to the sequence of the donor DNA. In each of three independent transformants, we have identified a SNP in a tRNA, either stabilizing the essential tRNAThrCGU at high temperature or switching the anticodon of tRNALysCUU or tRNAeMetCAU into CGU, which is apparently enough for in vivo recognition by threonyl-tRNA synthetase. LC-MS/MS analysis indeed indicated absence of significant mistranslation. Phylogenetic analysis showed that similar mutations have occurred throughout evolution and we suggest that stress conditions may have been a driving force for their selection. The low number of SNPs introduced by whole-genome transformation may favor its application for improvement of industrial yeast strains.

Dataset Information

Whole Genome Metabolism - Aster yellows witches'-broom phytoplasma (strain AYWB)

Publications

Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts.

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