Dataset Information

Whole Genome Metabolism - Neorickettsia risticii (strain Illinois)

ABSTRACT:

Whole Genome Metabolism of "Neorickettsia risticii (strain Illinois)"

This is a whole genome metabolism model of Neorickettsia risticii (strain Illinois).

This model has been automatically generated by the SuBliMinaL Toolbox and libAnnotationSBML using information coming from from KEGG (release 66, April 2013, accessed via the resource's web services interface) and, where relevant, augmented with metabolic pathway information extracted from MetaCyc (version 17.0, March 2013).

This model has been produced by the path2models project and is currently hosted on BioModels Database and identified by: BMID000000141670 .

Other models with the same genus include BMID000000010874 BMID000000010875 BMID000000010876 BMID000000010877 BMID000000010878 BMID000000010879 BMID000000010880 BMID000000010881 BMID000000010882 BMID000000010883 BMID000000010884 BMID000000010885 BMID000000010886 BMID000000073177 BMID000000073178 BMID000000073179 BMID000000073180 BMID000000073181 BMID000000073182 BMID000000073183 BMID000000073184 BMID000000073185 BMID000000073186 BMID000000073187 BMID000000073188 BMID000000073189 BMID000000073190 BMID000000073191 BMID000000073192 BMID000000073193 BMID000000073194 BMID000000073195 BMID000000073196 BMID000000073197 BMID000000073198 BMID000000073199 BMID000000073200 BMID000000073201 BMID000000073202 BMID000000073203 BMID000000073204 BMID000000073205 BMID000000073206 BMID000000073207 BMID000000073208 BMID000000073209 BMID000000073210 BMID000000073211 BMID000000073212 BMID000000073213 BMID000000073214 BMID000000073215 BMID000000073216 BMID000000073217 BMID000000073218 BMID000000073219 BMID000000073220 BMID000000073221 BMID000000073222 BMID000000073223 BMID000000073224 BMID000000073225 BMID000000141174 BMID000000011235 BMID000000011236 BMID000000011237 BMID000000011238 BMID000000011239 BMID000000011240 BMID000000011241 BMID000000011242 BMID000000011243 BMID000000011244 BMID000000011245 BMID000000011246 BMID000000011247 BMID000000074727 BMID000000074728 BMID000000074729 BMID000000074730 BMID000000074731 BMID000000074732 BMID000000074733 BMID000000074734 BMID000000074735 BMID000000074736 BMID000000074737 BMID000000074738 BMID000000074739 BMID000000074740 BMID000000074741 BMID000000074742 BMID000000074743 BMID000000074744 BMID000000074745 BMID000000074746 BMID000000074747 BMID000000074748 BMID000000074749 BMID000000074750 BMID000000074751 BMID000000074752 BMID000000074753 BMID000000074754 BMID000000074755 BMID000000074756 BMID000000074757 BMID000000074758 BMID000000074759 BMID000000074760 BMID000000074761 BMID000000074762 BMID000000074763 BMID000000074764 BMID000000074765 BMID000000074766 BMID000000074767 BMID000000074768 BMID000000074769 BMID000000074770 BMID000000074771 BMID000000074772 BMID000000074773 .

To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

SUBMITTER: Path2Models project

PROVIDER: BMID000000141670 | biostudies-other |

SECONDARY ACCESSION(S): 24180668

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever.

Lin Mingqun M Zhang Chunbin C Gibson Kathryn K Rikihisa Yasuko Y

Nucleic acids research 20090806 18

Neorickettsia risticii is an obligate intracellular bacterium of the trematodes and mammals. Horses develop Potomac horse fever (PHF) when they ingest aquatic insects containing encysted N. risticii-infected trematodes. The complete genome sequence of N. risticii Illinois consists of a single circular chromosome of 879 977 bp and encodes 38 RNA species and 898 proteins. Although N. risticii has limited ability to synthesize amino acids and lacks many metabolic pathways, it is capable of making m ...[more]

PMID: 19661282

Publication: 1/2

Similar Datasets

Project description:tRNAs are encoded by a large gene family, usually with several isogenic tRNAs interacting with the same codon. Mutations in the anticodon region of other tRNAs can overcome specific tRNA deficiencies. Phylogenetic analysis suggests that such mutations have occurred in evolution, but the driving force is unclear. We show that in yeast suppressor mutations in other tRNAs are able to overcome deficiency of the essential TRT2-encoded tRNAThrCGU at high temperature (40°C). Surprisingly, these tRNA suppressor mutations were obtained after whole-genome transformation with DNA from thermotolerant Kluyveromyces marxianus or Ogataea polymorpha strains, but from which the mutations did apparently not originate. We suggest that transient presence of donor DNA in the host facilitates proliferation at high temperature and thus increases the chances for occurrence of spontaneous mutations suppressing defective growth at high temperature. Whole-genome sequence analysis of three transformants revealed only four to five non-synonymous mutations of which one causing TRT2 anticodon stem stabilization and two anticodon mutations in non-threonyl-tRNAs, tRNALysCUU and tRNAeMetCAU, were causative. Both anticodon mutations suppressed lethality of TRT2 deletion and apparently caused the respective tRNAs to become novel substrates for threonyl-tRNA synthetase. LC-MS/MS data could not detect any significant mistranslation and RT-qPCR results contradicted induction of the unfolded protein response. We suggest that stress conditions have been a driving force in evolution for the selection of anticodon-switching mutations in tRNAs as revealed by phylogenetic analysis. Importance of the work In this work we have identified for the first time the causative elements in a eukaryotic organism introduced by applying whole-genome transformation and responsible for the selectable trait of interest, i.e. high temperature tolerance. Surprisingly, the whole-genome transformants contained just a few SNPs, which were unrelated to the sequence of the donor DNA. In each of three independent transformants, we have identified a SNP in a tRNA, either stabilizing the essential tRNAThrCGU at high temperature or switching the anticodon of tRNALysCUU or tRNAeMetCAU into CGU, which is apparently enough for in vivo recognition by threonyl-tRNA synthetase. LC-MS/MS analysis indeed indicated absence of significant mistranslation. Phylogenetic analysis showed that similar mutations have occurred throughout evolution and we suggest that stress conditions may have been a driving force for their selection. The low number of SNPs introduced by whole-genome transformation may favor its application for improvement of industrial yeast strains.

Dataset Information

Whole Genome Metabolism - Neorickettsia risticii (strain Illinois)

Publications

Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets