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Bacteriophage P22 mature virion capsid protein


ABSTRACT:

SUBMITTER: Wah Chiu 

PROVIDER: EMPIAR-10083 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Accurate model annotation of a near-atomic resolution cryo-EM map.

Hryc Corey F CF   Chen Dong-Hua DH   Afonine Pavel V PV   Jakana Joanita J   Wang Zhao Z   Haase-Pettingell Cameron C   Jiang Wen W   Adams Paul D PD   King Jonathan A JA   Schmid Michael F MF   Chiu Wah W  

Proceedings of the National Academy of Sciences of the United States of America 20170307 12


Electron cryomicroscopy (cryo-EM) has been used to determine the atomic coordinates (models) from density maps of biological assemblies. These models can be assessed by their overall fit to the experimental data and stereochemical information. However, these models do not annotate the actual density values of the atoms nor their positional uncertainty. Here, we introduce a computational procedure to derive an atomic model from a cryo-EM map with annotated metadata. The accuracy of such a model i  ...[more]

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