Dataset Information
Cryo-EM reconstruction of a modified human Adenovirus C5
Ontology highlight
ABSTRACT:
SUBMITTER: Patrick Ernst
PROVIDER: EMPIAR-10117 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control.
Nature communications 20180131 1
Most systemic viral gene therapies have been limited by sequestration and degradation of virions, innate and adaptive immunity, and silencing of therapeutic genes within the target cells. Here we engineer a high-affinity protein coat, shielding the most commonly used vector in clinical gene therapy, human adenovirus type 5. Using electron microscopy and crystallography we demonstrate a massive coverage of the virion surface through the hexon-shielding scFv fragment, trimerized to exploit the hex ...[more]