Dataset Information
Volta phase plate data collection facilitates image processing and cryo-EM structure determination
Ontology highlight
ABSTRACT:
SUBMITTER: Bruno P Klaholz
PROVIDER: EMPIAR-10156 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Volta phase plate data collection facilitates image processing and cryo-EM structure determination.
Journal of structural biology 20180111 3
A current bottleneck in structure determination of macromolecular complexes by cryo electron microscopy (cryo-EM) is the large amount of data needed to obtain high-resolution 3D reconstructions, including through sorting into different conformations and compositions with advanced image processing. Additionally, it may be difficult to visualize small ligands that bind in sub-stoichiometric levels. Volta phase plates (VPP) introduce a phase shift in the contrast transfer and drastically increase t ...[more]