Dataset Information
T. acidophilum 20S proteasome core movies obtained using Talos Arctica operating at 200 kV equipped with a K2 – image shift used for exposure target navigation
Ontology highlight
ABSTRACT:
SUBMITTER: Mark Anthony Herzik Jr
PROVIDER: EMPIAR-10186 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV.
Nature methods 20171009 11
Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules. ...[more]