Dataset Information
Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome
Ontology highlight
ABSTRACT:
SUBMITTER: Jean-Paul Armache
PROVIDER: EMPIAR-10287 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.
eLife 20190618
The SNF2h remodeler slides nucleosomes most efficiently as a dimer, yet how the two protomers avoid a tug-of-war is unclear. Furthermore, SNF2h couples histone octamer deformation to nucleosome sliding, but the underlying structural basis remains unknown. Here we present cryo-EM structures of SNF2h-nucleosome complexes with ADP-BeF<sub>x</sub> that capture two potential reaction intermediates. In one structure, histone residues near the dyad and in the H2A-H2B acidic patch, distal to the active ...[more]