Dataset Information
Cryo electron tomography of spinach leaf tissue
Ontology highlight
ABSTRACT:
SUBMITTER: Fei Sun
PROVIDER: EMPIAR-10302 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography.
Journal of structural biology 20210624 3
Cryo-electron tomography (cryo-ET) provides a promising approach to study intact structures of macromolecules in situ, but the efficient preparation of high-quality cryosections represents a bottleneck. Although cryo-focused ion beam (cryo-FIB) milling has emerged for large and flat cryo-lamella preparation, its application to tissue specimens remains challenging. Here, we report an integrated workflow, VHUT-cryo-FIB, for efficiently preparing frozen hydrated tissue lamella that can be readily u ...[more]