Dataset Information
Human pre-B spliceosome and U4/U6.U5 tri-snRNP
Ontology highlight
ABSTRACT:
SUBMITTER: Max Wilkinson
PROVIDER: EMPIAR-10307 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Mechanism of 5' splice site transfer for human spliceosome activation.
Science (New York, N.Y.) 20190411 6438
The prespliceosome, comprising U1 and U2 small nuclear ribonucleoproteins (snRNPs) bound to the precursor messenger RNA 5' splice site (5'SS) and branch point sequence, associates with the U4/U6.U5 tri-snRNP to form the fully assembled precatalytic pre-B spliceosome. Here, we report cryo-electron microscopy structures of the human pre-B complex captured before U1 snRNP dissociation at 3.3-angstrom core resolution and the human tri-snRNP at 2.9-angstrom resolution. U1 snRNP inserts the 5'SS-U1 sn ...[more]